The enzymatic reaction catalyzed by lactate dehydrogenase exhibits one dominant reaction path
نویسندگان
چکیده
منابع مشابه
Changes in protein architecture and subpicosecond protein dynamics impact the reaction catalyzed by lactate dehydrogenase.
We have previously established the importance of a promoting vibration, a subpicosecond protein motion that propagates through a specific axis of residues, in the reaction coordinate of lactate dehydrogenase (LDH). To test the effect that perturbation of this motion would have on the enzymatic reaction, we employ transition path sampling to obtain transition path ensembles for four independent ...
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The transition path sampling method previously applied in our group to the reaction catalyzed by lactate dehydrogenase was used to generate a transition path ensemble for this reaction. Based on analysis of the reactive trajectories generated, important residues behind the active site were implicated in a compressional motion that brought the donor-acceptor atoms of the hydride closer together....
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ژورنال
عنوان ژورنال: Chemical Physics
سال: 2014
ISSN: 0301-0104
DOI: 10.1016/j.chemphys.2014.02.018